The Effect of Hibiscus rosa-sinensis L. Extract on Improvement of Macrophage Phagocytosis Activity
Diabetes mellitus (DM) is a risk factor of tuberculosis, that hyperglycemia conditions causing impaired immune responses including phagocyte function so that it can facilitate M. tuberculosis infection. Therefore, it takes therapy to improve the immune system, one of them by using Hibiscus rosa-sinensis L. extract as immunostimulant. Aim:
To analyze the effect of extract H. rosa-sinensis L. on macrophage phagocytosis activity in animal TB-DM model. Method:
The research was an experimental used 9 male Wistar rats, 8 weeks-old, weight 200-230grams, divided into 3 treatment groups i.e. K (control), P1 (positive TB-DM), P2 (positive TB-DM treated with H. rosa-sinensis L. extract), and treated for 21 days. Animal TB-DM model was prepared with the administration of 100mg/kg nicotinamide (NA) and 65mg/kg streptozotocin (STZ), and injected with 1,5x105 CFU M. tuberculosis. Macrophage activity test was performed using intraperitoneal fluid that staining with Giemsa. Phagocytosis activity was determined based on the number of macrophage cells in 100 cells. The data were obtained from that test, then analyzed using Kruskal Wallis. Result:
The macrophage activity test showed that the mean macrophage activity in group K was 57 ± 2.082 cells, P1 group was 46 ± 2,517 cells, and P2 group was 82.33 ± 2,404 cells. The result of statistic analysis showed that H. rosa-sinensis L. extract was able to increase macrophage activity significantly (P = 0.027 ).
H. rosa-sinensis L. extract increase the activity of peritoneal macrophages phagocytosis activity in animal TB-DM model.
Keywords: Hibiscus rosa-sinensis L., TB-DM, macrophage.